Sementara itu, Valentino Rossi, yang di tes pertama mampu bercokol di urutan ketujuh harus bernasib sial saat sesi berikutnya.
Pembalap berjuluk The Doctor itu tercecer di posisi kesembilan dengan mencatat selisih 0,702 detik dari Quartararo.
Penampilan kurang mengesankan juga ditunjukkan juara bertahan Marc Marquez (Repsol Honda).
Sempat mengalami insiden hingga motornya rusak parah, pria asal Cervera menuntaskan hari kedua tes pramusim di urutan ke-14.
| No | Pembalap | Tim | Waktu | Lap |
| 1 | Fabio Quartararo | Petronas Yamaha | 1m 54.038s | 52/65 |
| 2 | Alex Rins | Suzuki Ecstar | +0.162s | 52/53 |
| 3 | Maverick Viñales | Monster Yamaha | +0.226s | 53/68 |
| 4 | Franco Morbidelli | Petronas Yamaha | +0.443s | 55/70 |
| 5 | Francesco Bagnaia | Pramac Ducati | +0.482s | 37/52 |
| 6 | Johann Zarco | Avintia Ducati | +0.527s | 55/56 |
| 7 | Joan Mir | Suzuki Ecstar | +0.574s | 23/52 |
| 8 | Andrea Dovizioso | Ducati Team | +0.624s | 36/60 |
| 9 | Valentino Rossi | Monster Yamaha | +0.702s | 22/52 |
| 10 | Takaaki Nakagami | LCR Honda | +0.721s | 30/43 |
| 11 | Aleix Espargaro | Aprilia Gresini | +0.724s | 26/33 |
| 12 | Danilo Petrucci | Ducati Team | +0.814s | 23/49 |
| 13 | Pol Espargaro | Red Bull KTM | +0.852s | 25/54 |
| 14 | Marc Marquez | Repsol Honda | +1.055s | 26/46 |
| 15 | Jack Miller | Pramac Ducati | +1.156s | 11/42 |
| 16 | Tito Rabat | Reale Avintia | +1.314s | 53/54 |
| 17 | Miguel Oliveira | KTM Tech3 | +1.562s | 20/53 |
| 18 | Iker Lecuona | KTM Tech3 | +1.606s | 45/53 |
| 19 | Alex Marquez | Repsol Honda | +1.687s | 22/48 |
| 20 | Brad Binder | Red Bull KTM | +1.892s | 19/57 |
| 21 | Cal Crutchlow | LCR Honda | +2.140s | 26/27 |
| 22 | Bradley Smith | Aprilia Test Rider | +2.369s | 49/52 |
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